Tour of Department Instrumentation

NMR

The Department has two 300 MHz NMR spectrometers. Both are located in M002. The Bruker DPX-300, shown in the first photo, routinely does a variety of 1D and 2D experiments using an inverse broadband probe. The older AM-300, in the second photo, is used for short duration experiments on 1H, 13C, 31P, 19F, using the Quad Nucleus probe.  Additional probes are available for both instruments.  All users must attend a training class before using the NMR spectrometers.

EPR

The Bruker EMX spectrometer provides X-band (9GHz) EPR on a variety of samples.

Mass Spectrometry

The Department has three mass spectrometers.  Finnigan LTQ, shown in the first photo, is a linear ion trap, with ESI, APCI, and nanospray sources.  It was installed in 2004.  The mass spectrometer is interfaced to a Finnigan Surveyor LC system.  The Bruker Biflex MALDI-TOF, shown in the second photo, provides soft ionization using the MALDI (matrix assisted laser desorbtion ionization).  It provides mass data on molecules with masses up to a few tens of  thousands of Daltons.  The Shimadzu QP-5050A GCMS, shown in the third photo was acquired in 2005.

SEM

The scanning electron microscope provides imaging capabilities with resolution down to tens of nanometers.  The SEM can also analyze x-rays emitted when an electron beam strikes a sample.  These x-ray spectra can provide qualitative and semi quantitative elemental analysis for atomic numbers > 10.

Analytical Ultracentrifuge

 The Beckman XL-I ultracentrifuge, shown below was installed in 2004.  It is has both absorption and interference optics for sedimentation velocity and equilibrium studies of proteins.

X-ray Diffractometer

A Bruker P4 single crystal diffractometer is located in the Department of Biomedical Engineering.  The instrument is maintained by the Department of Chemistry and is available to all Chemistry department researchers. A second diffractometer for powder samples is also available.

UV/Vis/NIR

T he Department purchased a Jasco V-570 spectrophotometer in 2003.  It has a wavelength range of  190-2500nm, Accessory for solid sampling, and a programmable temperature controller.

FTIR

The department has a number of FTIR spectrometers for research and teaching.  Shown here are the Nicolet Magna 760(left) and Mattson RS-1 (right).  There is also a  Thermo IR-100, used primarily in the organic teaching lab. We have sampling accessories for transmission, attenuated total reflectance and diffuse reflectance measurements of solids, liquids and gases.

Fluorescence Spectroscopy

Shown here is Cary Eclipse fluorometer, purchased in 2001.  It measures emission and excitation spectra over the range 190-900nm.
 
 

ICP AES

The inductively coupled plasma atomic emission spectrometer  generates emission spectra by heating samples in an argon plasma at 6000 degrees C.  Detection limits for many elements are in the low ppb range.

Additional Instrumentation

Additional  instrumentation is located in teaching labs.  It is available for research whenever it is not in use for teaching.  Included in this group are a variety of UV/Vis, FTIR, AA, CG, HPLC instrumentation.
 

One of two HP 1050 HPLC Systems from Analytical teaching lab

One of three HP 5890 Gas Chromatograph from the analytical teaching lab
 

Last Revised 1/17/07