Tour of Tufts Chemistry Department Instrumentation

NMR

The Department has two NMR spectrometers. Both are located in M002. A Bruker Avance III 500 was installed in 2009.  At the same time the older Bruker DPX-300 was upgraded with pulsed field gradients and a new probe.   The 500 is used exclusively for research, while the 300 is used for both research and teaching.  All users must attend a training class before using the NMR spectrometers.

Bruker AVIII 500
 

 

EPR

The Bruker EMX spectrometer provides X-band (9GHz) EPR on a variety of samples.


 

Mass Spectrometry

The Department has three mass spectrometers.  Finnigan LTQ, shown in the first photo, is a linear ion trap, with ESI, APCI, and nanospray sources.  It was installed in 2004.  The mass spectrometer is interfaced to a Finnigan Surveyor LC system.  The instrument is used for both small molecule and peptide samples. A Bruker Microflex MALDI-TOF was installed in February, 2009, and is shown in the second photo.  The instrument uses MALDI (matrix assisted laser desorption ionization) to provide mass data on peptides and proteins up to a few tens of thousand Daltons.   The Shimadzu QP-5050A GCMS, shown in the third photo was acquired in 2005. It is used forvolatile samples up to about 500 Daltons.

            

 

Analytical Ultracentrifuge

 The Beckman XL-I ultracentrifuge, shown below was installed in 2004.  It is has both absorption and interference optics for sedimentation velocity and equilibrium studies of proteins.

 

Single Crystal X-ray Diffractometer

The Bruker Quest D8 single crystal diffractometer was a significant addition to Chemistry department instrumentation in 2013.  It can determine molecular structure of any compound for which well formed crystals can be grown.

 

 

UV/Vis/NIR

The Department purchased a Jasco V-570 spectrophotometer in 2003.  It has a wavelength range of 190-2500nm, It has an integrating sphere accessory for solid sampling, and a programmable temperature controller.




FTIR

The department has a number of FTIR spectrometers for research and teaching.  The left photo shows Nicolet Magna 760 used in the PChem teaching lab, and for research. The second and third photos show the Thermo IR-100 and Thermo IS-5, used primarily in the organic teaching lab. We have sampling accessories for transmission, attenuated total reflectance and diffuse reflectance measurements of solids, liquids and gases.

 

    


 

Fluorescence Spectroscopy

Shown here is Cary Eclipse fluorescence spectrometer.  It measures emission and excitation spectra over the range 190-900nm.
 


 

ICP AES

The Leeman Labs Prodigy inductively coupled plasma atomic emission spectrometer, acquired in 2010, generates emission spectra by heating samples with an argon plasma at 6000 degrees C.  It is used for quantitative determination of most metals and some non-metals.  Detection limits for many elements are in the low ppb range.



 

Additional Instrumentation

Additional instrumentation is located in teaching labs.  It is available for research whenever it is not in use for teaching.  Included in this group are a variety of UV/Vis, FTIR, AA, CG, HPLC instrumentation.
 

One of two Agilent 1260 HPLC Systems in the newly renovated (2016) Analytical teaching lab


 

One of two Agilent 7890 Gas Chromatograph in the newly renovated (2016) analytical teaching lab

 
 

 Additional UV Vis spectrophotometers such as the Genesys 10S (left) and Cary 60 (right) can be quickly relocated to various teaching labs as needed.

 

 

Last Revised 10/20/16

By David Wilbur